FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4631343
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG24900.060.58536
CTATAGG22150.060.206116
CAGATAG23050.059.525055
GATAGGT26700.059.2500047
CGGATAG15550.058.973325
CTGATAG24600.058.3355645
CGTATAC6750.058.0762945
CGTATAG15650.057.7018935
GTATAGG26700.057.680736
CGCGATA9350.057.6492654
CCTATAG15100.057.022055
GGATAGT25700.056.9288646
TTATAGC36250.056.8717586
TCTAGCG18000.056.7768827
TGATAGG34900.056.5643776
GATAGTC15300.056.502387
GATAGGG24250.056.1434487
GCTAGCG16550.056.0414167
GGATAGC14850.055.8613246
GGGATAG23500.055.2574355
Produced by FastQC (version 0.11.5)