FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265320
Sequences flagged as poor quality0
Sequence length76
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAC1850.060.543585
GGATAGG22450.058.4670756
TGATAGG19400.058.457436
CTATAGG11700.058.337156
GATAGGG24100.057.94637
GGTATAG17650.057.3116535
CCGATAC2450.057.1457185
CGATAGG11150.057.133786
GATAGGT18350.057.030217
GGATAGT19350.056.9804736
CGGATAG11150.056.818995
GCGATAG11550.056.6695065
CCCGATA5000.056.0028084
GATAGTG20350.055.8970347
CGATATG11200.055.628646
GTATAGG20800.055.5324826
ATAGGGG18550.055.471938
GATAGGA19850.055.365477
GTGATAG17450.055.360945
GGGATAG20200.055.2750435
Produced by FastQC (version 0.11.5)