FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3187280
Sequences flagged as poor quality0
Sequence length76
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATAG17650.058.7025
GGATAGT17450.058.171266
CGGATAG9250.057.896945
GCGATAG9950.057.341685
GGATAGG19300.057.3106966
GATAGGG18750.056.9320457
CGATAGG9400.056.2283066
CGCGATA6450.055.8963474
CCCGATA4400.055.6866764
GATAGGT17600.054.8851247
CGATAGT10150.054.487516
CAGATAG12100.054.0956275
GGGGATA15450.053.920564
ACGATAG8800.053.6978655
GCGATAA12350.053.2839975
GCTGATA13700.053.143324
CTATAGG9100.053.081556
TGATAGG18350.052.8383946
GCGGATA11600.052.5045784
ATAGGGG13900.052.3737378
Produced by FastQC (version 0.11.5)