FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537033
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG27580.10870966203435273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGGA36300.060.8382537
GGATAGG16850.060.656836
TGATAGG30750.060.3291936
TCCCGTC9200.059.725159
CGCGATA5900.058.7326974
CTATAGG13550.058.6385656
CCCGTCC11500.056.91007610
CCCGATA4800.056.878764
GATAGGT14250.056.7350047
GATAGTC11050.056.6957
CCGCTAC2300.056.3080755
CGTATAG9700.056.2923855
CTAGGAC8300.056.0814178
GCTCCCG3500.055.998197
GATAGGG17100.055.8753857
CGGATAG10200.055.5919155
GCGATAA8950.055.5344055
CTGATAG23900.055.5057645
GTAGGAC8900.055.4465528
CGTATAC3800.055.2668155
Produced by FastQC (version 0.11.5)