FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPHK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPHK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2656881
Sequences flagged as poor quality0
Sequence length76
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT9200.057.8276446
CGCGATA5750.057.8276374
GCGATAA9200.057.0667535
CGGGATA9100.055.7707334
CGTGATA9650.055.131024
GGATAGG19200.055.0535666
GATAGGG18800.054.918597
GCGATAG9150.054.7009245
CAGATAG11300.054.2049985
GCCGATA5150.053.690764
ACGATAG7250.053.5876465
GGGATAG19000.053.4224855
TGCGATA8600.053.3153884
GGATAGT18850.053.290566
CGGATAG7050.053.1219945
GATAGGT17300.053.0042087
TGGATAG21400.052.6649675
GTATAGG16050.052.5559276
CGATAGG8400.052.501416
CCCGATA4200.052.501414
Produced by FastQC (version 0.11.5)