FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPHK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPHK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998016
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC2000.059.5001265
CGTATAG12200.056.5165145
GGATAGG19400.056.4701356
CCTATAG8050.055.652295
TATAGGG22600.055.440757
GTATAGG21050.055.0366676
GCGATAG12400.054.7581835
CGGGATA10050.054.6767354
CGATAGT12200.054.5092246
CGGATAG10600.054.481255
GGATAGT20900.054.426866
CCCGATA4850.054.123834
GATAGGG21150.054.1117867
GCGTATA13100.053.969584
GATAGGT21200.053.8190737
CCCGTCC7100.053.73071710
ATAGGGG14000.053.4983378
GGGATAG19400.053.2217645
GGTATAG19100.052.9582335
CAGATAG13900.052.8778085
Produced by FastQC (version 0.11.5)