FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPHK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPHK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603289
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC3850.060.912155
GGTATAG18100.060.7212835
GATAGGG16900.059.8504757
CGATAGG8150.059.266786
CCTGATA6500.059.2337464
CGATAGT9400.059.2051056
GTATAGG18150.058.8183826
CCTATAG7100.058.6649175
CGCGATA7250.058.4167254
GGGATAG17400.058.13515
CCTATAC5850.058.03715
GGATAGT15500.058.035176
GGATAGG19650.057.7128226
GCGTATA10750.056.9796074
GATAGGT15550.056.943827
CGGGATA10150.056.8994064
CCGATAC2750.056.0028085
TATAGGG19750.055.998517
GCGATAG9200.055.9267235
CTATAGG11700.055.3446586
Produced by FastQC (version 0.11.5)