FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPHK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPHK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4101285
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC7600.063.5546045
CGGATAG14000.060.7518845
CCTATAG14900.060.3715
CAGATAG24450.060.2677155
GATAGTC13700.060.03477
CTATAGG22850.059.4329156
TATAGGC27400.059.26837
GGGATAG19200.059.064335
CTGATAG24950.059.0599485
GCGATAG14650.059.012075
GGATAGG21250.058.9665376
TTATAGC34250.058.863146
GGATAGC11900.058.5312276
CTATAGT19200.058.335146
GTATAGG22900.058.0804066
ATTATAG42500.057.978275
CGATAGT14500.057.932836
GGATAGT20750.057.351186
CGTATAG15000.056.9350975
GCTAGCG17400.056.923587
Produced by FastQC (version 0.11.5)