FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPHK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPHK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756563
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTC8250.059.818217
CGATAGG9200.059.3489346
CGGATAG9700.059.176365
CCTGATA6100.058.525684
GCGATAA10300.058.447695
TATAGCG9250.058.2702987
GATAGGT21250.057.97657
GGATAGT16500.057.9101686
GGATAGG21450.057.6001436
GGGATAG20950.056.970045
GATAGGG18850.056.259987
CGCGATA6550.056.1079184
GTATAGG17900.055.72736
TGATAGG17550.055.0437626
CGATAGT10450.054.9292536
GATAGTG20750.054.3132827
CTATAGG9800.054.2867286
GCGATAG10400.054.1837045
ATAGGGG14100.053.8643048
CGTATAG8600.053.721935
Produced by FastQC (version 0.11.5)