FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_LPHK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_LPHK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4517743
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG24250.060.9065446
CAGATAG24900.060.300545
CTGATAG26750.060.186255
CGGATAG14900.059.4288675
CGTATAG17650.058.894535
TTATAGC35150.058.4488566
GATAGGT25400.058.4245457
TCTAGCG19350.058.4231267
CCTATAG15750.058.2215735
CGTATAC6700.057.984435
CCGATAC3950.057.59435
GGATAGC13150.056.9575466
GATAGGA40250.056.3472067
TGATAGG37500.056.1860476
TATAGGC26400.056.0789267
GTATAGC18250.055.9993866
GATAGTC16100.055.8689467
TATAGTG37600.055.8504457
GTATAGG28200.055.478116
CTATAGT22850.055.4479646
Produced by FastQC (version 0.11.5)