FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPLK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPLK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729092
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG9850.061.471785
CGGGATA9500.058.2102364
GGATAGT19700.057.5631686
CAGATAG12700.057.0469635
GTATAGG16150.056.77996
CGATATG12350.056.113086
GATAGGT18100.056.0760427
CGATAGT11300.056.061676
CGATAGG9450.055.555286
GCGATAG11450.055.3272365
AGGATAG12150.055.02035
GCCGATA6400.054.687234
GATAGTC10000.054.598737
CGCGATA7250.054.068694
TGCGATA11600.054.008354
GCGATAA10700.053.971695
TATACGG6300.053.887637
GCGGATA11350.053.6561164
TATAGCG8700.053.5044987
TGATAGG16650.053.3931276
Produced by FastQC (version 0.11.5)