FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPLK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPLK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739127
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG17350.059.3124546
TTATAGC18900.059.078166
CGTATAG9900.059.0444835
CGGATAG9400.058.0891235
CGCGATA5800.057.935044
CTATAGG12750.057.3765146
GATAGGG17700.057.3433657
GCGTATA9700.057.0142484
TGATAGG19700.056.5013666
ACGATAG8550.056.0857435
GGATAGT18600.056.0791446
TATAGGC15550.056.0437747
CACGATA7600.055.7275354
TATAGGT23050.055.5736057
CTGATAG14750.055.5292635
GGATAGG15700.055.513396
GATAGTC11750.055.403037
TATAGGG21200.055.1402857
CCGCTAC3050.055.0857735
CGATAGT11300.054.8267976
Produced by FastQC (version 0.11.5)