FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPLK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPLK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552675
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG22600.057.768026
CGGATAG11050.057.332835
CGCGATA6600.057.2752384
GCGATAA14250.056.9849555
CCCGATA5550.056.7592434
GCGATAG11750.055.7045675
GGATAGT22900.055.4827846
CGGGATA12200.055.3712774
ATAGGTC9550.054.9731378
GATAGTC11850.054.9360247
GGTATAG23050.054.6661725
CGATAGG12000.054.544066
CGATATG16000.054.252386
CAGATAG16400.053.9962655
GATAGGT26750.053.513357
TATAGCG11850.053.4592487
TGCGATA15500.053.2926564
CGTATAC4100.052.9291465
TATACGG8750.052.799347
CCTATAG9250.052.59695
Produced by FastQC (version 0.11.5)