FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPLK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPLK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3513125
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGATAG22700.062.754995
TGATAGG28350.062.3482366
CTATAGG17250.061.8866046
CGTATAG12100.061.0346955
GGATAGG16700.060.361766
TTATAGC29050.060.243446
CGGATAG13200.059.9258545
CCTATAG13500.059.8904955
ATTATAG34250.059.7826235
CGATAGT11900.059.7083366
CAGATAG18250.059.645445
GGATAGC10500.059.3357666
GATAGTC13350.058.7264987
CGTATAC5850.058.6340565
GATAGGG16200.058.549297
TATAGGC22700.058.4360287
GTATAGC14050.058.2942056
GCGTATA9850.058.2748454
CGCGATA7150.058.2524834
ACGGATA12100.058.1412284
Produced by FastQC (version 0.11.5)