FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPLK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPLK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272621
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT24100.057.6600726
CGGGATA10250.057.37034
GGGATAG22050.056.671065
CGGATAG10950.056.2600675
CGATAGG11500.056.0043376
GGATAGG24350.054.624366
GATAGGT23500.054.0630657
GGTATAG20900.053.7601475
GATAGGG22650.053.31057
GTATAGG21400.053.1583256
CGCGATA7500.052.737424
CGTATAG10950.052.4241525
CCGTATA6500.052.2348144
GCGATAG12500.052.0840345
ATAGGCG5400.051.8511128
TATAGGC13400.051.7156877
TATAGGT30150.051.6576427
CAGATAG16150.051.5829435
CGATATG15450.051.4279336
CGCTATA9900.051.2665984
Produced by FastQC (version 0.11.5)