FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPLK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPLK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136330
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA7350.059.048964
GGATAGG20200.057.3527956
CGGATAG10100.055.4468085
CGGGATA11400.055.2644124
CCTATAG7050.055.107645
GCGATAG12800.054.962195
GATAGGG22400.054.8423777
GATAGGT23400.053.8448077
GGGATAG22500.053.667895
GCGATAA12500.052.6411975
GGATAGT22400.052.5011986
CCGCTAC2700.051.853035
CGATAGT13300.051.843296
GCCGATA8200.051.647524
TATAGGG23000.051.5856677
GGATAGC9650.051.5037656
CGTATAG10550.051.0912135
GGTATAG19700.050.991015
TGATAGG23250.050.8828776
CAGATAG16050.050.811135
Produced by FastQC (version 0.11.5)