FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011484
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG9850.061.116625
CAGATAG13300.057.3682985
CGGGATA10750.056.976624
GTATAGG19050.056.7724766
GGATAGT19750.056.7096826
GCGATAG10250.056.6828085
CGCGATA6650.056.3156664
TGATAGG21450.055.8050046
CGTATAG11300.055.442365
CGATAGG9200.055.163846
CGATAGT11650.054.979346
CTATAGG13950.054.947036
GATAGGG20850.054.7241027
CTGATAG15000.054.3665545
CGTGATA12300.054.0649264
GGTATAG18850.054.031725
GCGTATA13050.053.9079324
GATAGGA22600.053.5839587
GCGATAA11200.053.4373865
TATAGCG10350.053.4298367
Produced by FastQC (version 0.11.5)