FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3495064
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT25800.057.247796
GGATAGG26150.055.6785166
CCTATAG8050.054.3475725
CTATAGG13950.054.1932956
CGGGATA11950.054.1838464
CGCGATA8300.053.975654
GCGATAA13000.053.8458985
GGTATAG22200.053.603355
GTATAGG24250.053.546146
GATAGGT25050.053.0928047
CGGATAG11900.052.9409265
CCCGTCC4150.052.28816610
TATAGCG11650.052.2736857
GATAGGG23850.051.801957
GGGATAG27400.051.733335
CGATAGT14750.051.7285736
CCCGATA5300.051.5091934
CCGCTAC3200.051.406015
TATAGGC15250.051.179357
GCGATAG13550.051.143675
Produced by FastQC (version 0.11.5)