FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2893646
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10800.060.2796675
GATAGTC8950.056.7027447
GGATAGT18350.056.650286
CGCGATA6100.055.6574824
GCGATAG12050.055.4789165
GTATAGG17850.055.295856
CGATATG12100.055.249676
GGTATAG17050.055.0163885
CGATAGT11200.055.0017246
CCCGATA5700.054.6508374
GGATAGG21800.054.5888676
GCGTATA12000.054.5433774
GGGATAG20100.054.1559265
ATAGGGG13400.054.066068
GATAGGG18650.054.0471467
TGCGATA12500.054.041694
CGGGATA11050.053.847844
GCGATAA10600.053.4922455
CGTATAG10450.053.255265
GATAGGT21050.052.8730247
Produced by FastQC (version 0.11.5)