FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2538645
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC1800.058.3357665
CGCGATA5300.058.1156274
GGATAGT18850.056.0766076
CGGATAG9550.055.709135
GGTATAG16350.054.803515
CGATAGT11500.054.784896
GGATAGG21200.054.6485026
CGGGATA9800.054.2879754
GCGATAA10750.054.0487675
CGTATAC2600.053.84845
GCGATAG10500.053.3355565
GATAGGT20250.052.8879667
GGGATAG18400.052.8826335
CAGATAG11200.052.8146935
GTATAGG19700.052.5910226
TATAGGG22200.052.3414277
CTGATAG12700.052.0887875
CCTATAG5150.051.6526345
GATAGGG20300.051.5508237
CGCTATA7150.051.400744
Produced by FastQC (version 0.11.5)