FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3135483
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGG10950.059.134746
CGGATAG10300.059.127595
CGCGATA6500.057.6167224
CGGGATA9950.057.3380174
GGTATAG18000.056.1957475
GCGATAG11800.056.0606235
GATAGTC12000.056.000417
GTATAGG20650.055.42596
GGATAGT20150.055.4116026
CGTATAG10000.055.3012855
CAGATAG14450.055.2261965
AGGATAG15450.054.5967375
GATAGGT22450.054.098397
CGATATG15300.053.9890446
GCCGATA7550.053.776084
TATAGGC14650.053.7546547
GCGATAA11950.053.599575
TGATAGG21150.053.122656
CGATAGT12350.052.9980286
CCGATAC3050.052.788115
Produced by FastQC (version 0.11.5)