FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_wt-0.5_HPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_wt-0.5_HPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624392
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA6500.058.696544
GGATAGG18250.056.7712176
GGTATAG16300.055.832255
GATAGGG17100.055.4676137
CTATAGG11300.055.1367236
GGATAGT18500.055.0580256
GGGATAG17350.054.874275
CGGATAG9450.054.8187685
CCCGTCC3950.054.04937710
ATAGGGG11150.053.9897848
CGGGATA8900.053.8802874
GTATAGG16250.053.203846
GCGATAG11000.052.821995
GCGCTAC2850.052.8108255
CGATAGT12450.052.0117846
TATAGGG21300.051.7603537
GTATAGC7600.051.5826646
TGATAGG19900.051.536386
CCCGATA4900.051.432284
CCTATAG6950.051.3706135
Produced by FastQC (version 0.11.5)