FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9912471
Sequences flagged as poor quality0
Sequence length76
%GC64

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACCCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG586050.5912249327135484No Hit
GCGACCCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG575240.5803194783621561No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG311520.31427078071653375No Hit
GCGACCAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277860.2803135565289422No Hit
AAAAAACGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG253320.25555686367203495No Hit
CGACCCCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG221950.22390986061901216No Hit
CGACCCCGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152530.15387686884531618No Hit
GGGGGGAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138380.1396019216600987No Hit
CTCAACCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG125120.12622483334377474No Hit
CAAAAACGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114650.11566238125690356No Hit
CGGAAGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103870.10478719181120429No Hit
CGGAAGAGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103510.1044240129428878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG282200.065.870946
CGACCCG76600.065.858312
ACGCGGG256400.065.632816
GACCCGC66250.065.141923
CGCGGGG1169650.063.942287
GACGCGG67300.063.5519035
TTCGCGG75550.063.5148625
TGCGCGG65200.063.3978775
CCGCGGG311950.062.752996
AACGCGG111200.062.7299465
CTCGCGG70400.062.4935235
CCGACCG15850.061.8449332
ACCCGCG82650.061.488824
TCCGCGG58450.061.25815
GTCGCGG87050.061.2355355
ACCCCGG71850.061.2338873
ACCGCGG55800.061.1565255
AGCGGGG527250.060.9680257
ACCCCGC30850.060.699063
GAGCGGG240950.060.370286
Produced by FastQC (version 0.11.5)