FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474094
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC2700.060.92715
CGATAGT8100.058.334466
CGCGATA6200.057.0172354
CGGATAG9150.056.2305955
CCTATAG7000.056.0010835
GGATAGT16100.055.8706446
GTATAGG16300.055.82936
TGATAGG17150.055.5112766
GCGATAG9350.055.402145
CAGATAG11650.055.280045
CGATAGG8250.055.152586
CGGGATA8200.055.0620424
GATAGTC8200.055.0598147
GATAGGG16550.054.772267
CGTATAG9800.054.6439135
ACGATAG6400.054.1416745
CCCGATA5250.054.001044
GTGATAG16000.053.5947885
CTGATAG12250.053.4296045
TATACGG5050.053.365217
Produced by FastQC (version 0.11.5)