FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2604983
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10550.060.3808565
CGATAGG10000.059.8516926
GGATAGT17650.057.905326
CGCGATA6600.057.8046654
CGATAGT10300.057.768626
GCGATAG9900.057.627895
CGTATAG9100.057.3093155
GTATAGG16200.057.25476
GATAGTC10450.056.9383167
CCCGATA4700.055.8526424
ACGATAG8900.055.8442735
CAGATAG11300.055.7537885
CGGGATA9900.055.506624
GATAGGA20500.055.4883047
GATAGGT16550.055.408367
GGTATAG17900.055.1412245
CCGATAC2300.054.7841535
GCGATAA10650.054.555535
GGATAGC7150.054.33726
TATAGCG8250.054.3035247
Produced by FastQC (version 0.11.5)