FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3218942
Sequences flagged as poor quality0
Sequence length76
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT25650.057.858736
GGATAGG18900.057.2251746
CTATAGG11450.056.858826
CGCGATA5050.056.8337254
GGGATAG19800.056.3909155
GCGATAA10700.055.6094745
CGGGATA9100.055.386614
GATAGGG18500.055.0543257
GCGATAC4100.054.636115
CGATAGG9100.054.6181956
CGTATAC3400.054.5607835
ATAGCGG5200.054.5194978
GGTATAG18800.054.177485
ATAGGGG13250.054.1512078
GCGTATA8600.054.1298564
TATAGGG23150.054.1255347
GCCGATA7050.054.115424
TTATAGG33250.054.002786
CAGATAG11800.053.9849935
GGCGATA11050.053.8480954
Produced by FastQC (version 0.11.5)