FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194303
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATAG13600.062.0256735
CCTATAG7950.061.1985325
CTATAGG10450.060.6269266
GGATAGG13650.059.7484366
CGTATAC3700.059.598075
TTATAGC17350.059.5149086
CACTATA10900.058.7648934
CGGATAG8600.058.201075
CCGCTAC2250.057.5589075
ATAGTGC6650.057.3691528
TCTAGCG10150.057.2421047
GATAGGG13350.057.154287
GGATAGT12650.057.0006686
TATAGGC14750.056.949877
CGTATAG8450.056.748875
CTATAGT10450.056.607466
GCTAGCG9300.056.452337
CGATAGT7450.056.380416
CCGTATA6350.056.223754
GATAGGT13550.056.0523727
Produced by FastQC (version 0.11.5)