FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPHK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPHK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903278
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC4650.060.217875
GTATAGG17950.059.083546
CCGATAC3450.058.843335
CGGATAG9800.058.574175
CGTATAG12050.058.3844765
CGATAGG11600.057.9337436
CGGGATA10300.057.7696884
GTGATAG18700.057.4625785
CAGATAG13450.057.251755
CGATAGT11750.057.194166
GCGATAG10450.056.605525
GTATAGC9350.056.5267076
CGCGATA6450.056.4367454
GATAGGA26700.056.3657957
GCGATAA12650.056.1686365
TATACGG7800.056.08857
GGATAGT17650.055.9232986
TGATAGG21850.055.746316
ACGATAG10800.055.7433475
GATAGTC11550.055.4533167
Produced by FastQC (version 0.11.5)