FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPHK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPHK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187039
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC6700.064.255565
TTATAGC35100.061.7261436
CCTATAG14950.061.5736585
CTGATAG20750.060.724625
GTATAGG24400.060.104896
CTATAGG19700.060.053196
GGATAGG21700.060.002436
GATAGGT20050.059.8755877
GGATAGC11600.059.7437976
CGCGATA8750.059.2016874
CAGATAG22750.059.078615
TCTAGCG22000.058.863917
CGTATAG16550.058.5817265
TATAGGC27000.057.685457
CGGATAG14200.057.67775
CTATAGT19750.057.5972636
GCGTATA14000.057.5016374
ATTATAG41100.057.483395
GTATAGT24250.057.3012856
TATAGCC9300.057.2045637
Produced by FastQC (version 0.11.5)