FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPHK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPHK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327978
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC3550.060.142915
CGATAGG9650.059.12126
CGCGATA5550.058.650664
CGGATAG7700.058.1838155
ACGATAG7400.057.704695
CGATAGT9050.056.8527836
GGATAGT15250.056.4609576
CAGATAG11350.056.4336555
GCGATAG10800.055.7426575
GATAGTC7900.055.3792767
CCTATAG6850.055.1843835
GATAGGT14800.055.100887
GATAGGG16750.054.7457967
TATAGGC9700.054.1232457
GCGATAA8300.053.556065
TGATAGG16350.053.5186586
GTATAGG14400.053.474066
CGTATAG8000.053.3768355
GTGATAG15300.053.3024865
CTGATAG10850.053.2276345
Produced by FastQC (version 0.11.5)