FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPHK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPHK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180514
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG17850.064.707796
CCGCTAC2500.064.401895
TGATAGG33850.063.901446
CTATAGG19000.062.8176356
CTGATAG27600.062.646775
GGATAGC9750.062.4633756
GGGATAG14600.062.3306055
GATAGGA41500.061.985887
ATTATAG33250.061.8965645
TTATAGC28350.061.853676
GATAGTC13850.061.658597
CGATAGT10850.061.6147166
GTCTACC4550.061.5402686
CGTATAG13500.061.446255
CCTATAG11750.061.3635065
CGGATAG11050.061.133025
GTATAGG20600.060.827036
ATATGCG19500.060.306628
CGTATAC6700.060.0763935
GTCGATA11600.060.044874
Produced by FastQC (version 0.11.5)