FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPHK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPHK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592247
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA10400.057.8860974
CGGATAG9950.057.6899225
GGATAGT16150.057.6496476
CGCGATA6300.057.223694
ACGATAG6450.056.9782035
CAGATAG12450.055.6640745
CGATAGT9350.055.403566
CGATAGG10200.055.247586
CCGTATA6000.054.8347364
GTATAGG16900.054.0556836
GGTATAG16400.053.7818685
GCGATAG10000.053.551375
GCGTATA10000.053.551374
CCCGATA4650.053.442234
AGGATAG11000.053.1377225
CCCGTCC2550.052.158210
GCGATAA11300.052.0367285
GATAGGG18300.052.0211837
CGTATAG9900.051.9710275
CTGATAG12950.051.893225
Produced by FastQC (version 0.11.5)