FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_LPHK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_LPHK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4164841
Sequences flagged as poor quality0
Sequence length76
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT29050.059.523466
GGATAGG21250.059.2994846
GATAGGT26950.058.051637
GGGATAG18300.056.99975
GATAGGG18150.056.693917
TATAGGG24150.056.37657
CGCGATA8200.055.91974
CGATAGT15900.055.2565776
CGGATAG11850.055.237075
GGTATAG22600.054.9828535
GTATAGG23300.054.6830646
CGATAGG10450.054.5982486
CTGATAG16750.054.3332755
GGCGATA11850.054.0555274
CGGGATA11700.053.8510324
CGGGTAC1500.053.6715245
TTATAGG38200.053.51276
GCGATAG12300.053.500785
CTATAGG13450.053.3505556
CGTATAG11750.053.3239755
Produced by FastQC (version 0.11.5)