FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPLK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPLK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827587
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG6700.060.598965
CGCGATA4250.059.2960134
GTATAGG12450.057.9135066
CCCGTCC4300.057.78938710
CGATAGG6600.057.2745636
TGATAGG13900.057.1601076
ACGGATA5850.056.241124
CGGGATA5800.056.122494
CCCGATA3000.056.0017934
TATAACG6000.055.998737
GATAGGT11250.055.998737
GCGATAA7700.055.9108855
CCTATAG6100.055.657525
CGTATAG6500.055.4633185
CAGATAG7650.055.361255
GATAGGG10700.055.279127
CCTGATA4700.055.108154
TATAGCG7050.055.1051337
TATACGG4400.054.8851247
GATAGTC7150.054.8239337
Produced by FastQC (version 0.11.5)