FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPLK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPLK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2966137
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG9350.057.2754555
CGCGATA6100.056.805994
CGGATAG10250.056.685635
GGATAGG21450.056.6227466
CCCGTCC8800.056.07843810
GGTCCCC1200.055.4155737
CGGGATA10300.055.0511674
GATAGGA26300.054.6947367
GATAGGG19950.054.560337
GATAGGT19800.054.4433677
GCGATAA11700.054.1478845
GGATAGT22000.053.616196
CGCTACC2100.053.335886
GGTATAG17800.053.2890555
TATAGGG24000.052.9364557
GCGATAG12350.052.715065
GTATAGG19150.052.6395846
CAGATAG14250.052.5639085
CCGCTAC2600.052.5025065
AGGATAG15450.052.3325965
Produced by FastQC (version 0.11.5)