FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPLK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPLK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3067554
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10200.058.676555
CGATAGT12750.058.196166
CGCGATA8000.058.1875844
CGGGATA10900.057.4771424
GATAGCC4300.056.97597
GCGATAA11100.056.7568365
GGATAGT22050.056.1905566
GTATAGG17500.055.800086
GCCGATA7600.055.7237634
GCGTATA11000.055.3637164
ACGATAG8100.054.4445155
CAGATAG15000.054.366745
GATAGGG17350.054.264327
CGCGTAT2600.053.846233
AGGATAG14150.053.6749885
CCTGATA9000.053.666744
CGTATAG10450.053.5885935
CGATATG12650.053.3992846
GATAGGT22300.053.2054867
CGATAAC6250.053.2000736
Produced by FastQC (version 0.11.5)