FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPLK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPLK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3877131
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC6700.065.304975
TTATAGC35050.062.716626
CTGATAG26500.062.21375
TGATAGG32200.062.1800656
CTATAGG20650.062.040046
CAGATAG25800.062.0022625
GATAGTC12200.061.9669537
GTATAGG23800.061.623746
CTATAGT13850.060.9085546
CTAGGAC9150.060.818638
CGTATAG14800.060.783045
GTATAGC16850.060.658826
GGATAGC12650.060.598886
TCTAGCG20750.060.5539637
ATTATAG38400.060.435675
CCTATAG13650.060.262375
CGATAGT12850.060.2005126
GGATAGG21700.060.0059366
CGGATAG14600.059.937445
GGATAGT17900.059.8383186
Produced by FastQC (version 0.11.5)