FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPLK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPLK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2805022
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10750.057.627795
CAGATAG12850.057.47075
CGCGATA7350.057.142744
CGGGATA11000.056.636254
GGATAGT19500.055.4614226
CGATATG12100.055.247826
CGATAGG11200.054.687396
CCGTATA6850.054.671424
GTATAGG18350.054.5502976
ATAGTGG13250.054.4140138
CCGATAA8150.054.1103175
CGATAGT10350.054.106176
GCGATAA9750.053.8460435
CCTATAG9250.053.7296185
GATAGTC11800.053.6853757
AGGATAG13300.053.4209445
GCCGATA6950.053.3811874
GATAGTG19550.053.3493237
GATAGGG20600.053.1785557
CGTATAG10350.053.0916825
Produced by FastQC (version 0.11.5)