FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPLK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPLK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2905811
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC4700.061.066025
GGATAGG19900.060.32886
CTATAGG14850.059.631776
CAGATAG13350.059.5152475
GTATAGG20200.059.432836
CGCGATA6950.058.419364
CGCTAAC1500.058.335436
CGATAGT11350.058.284036
CGGATAG11300.057.9224325
GCGATAG11250.057.8687485
GATAGGA32500.057.3981137
CGCTACC2650.056.7944956
GGGATAG17750.056.790775
TATAGGT22600.056.524697
GCGATAA12100.056.4069865
CCCGTCC9500.056.3675410
TCCCGTC8650.056.241899
TGATAGG25200.055.557556
GATAGGT18150.055.3425257
CCTATAG8100.055.310635
Produced by FastQC (version 0.11.5)