FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3124710
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGT19950.058.6003846
CGGATAG11350.058.2848855
CGGGATA11050.056.6996964
GATAGTC10700.056.5898327
ACGATAG9550.056.4426425
CGATAAC6300.055.559256
GTATAGG19150.055.1994936
CGATAGG9650.054.770486
GGTATAG20350.054.351645
CGCGATA8250.054.3057754
AGGATAG15050.053.9562155
CGATAGT11950.053.89486
CGTATAC4850.053.4047625
GGGATAG21750.053.267065
GATAGGT21300.053.240427
CGATAAT14150.053.183756
CGTATAG10600.053.163065
GCGTATA11200.053.127684
CCTATAG8250.053.032985
CAGATAG14000.053.0026745
Produced by FastQC (version 0.11.5)