FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3234191
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG12600.059.445065
CCCGTCC8050.059.12921510
CGCGATA6050.059.008884
GTATAGG20400.057.1329466
GCGATAA11950.057.1135675
GCGATAG13450.056.7292145
CCCGATA4150.056.506614
TGATAGG28750.056.0005846
GGATAGG21250.055.8358766
CCGCTAC1950.055.64165
TCCCGTC7700.055.4534039
CCTATAG8400.055.0005725
CGCTACC2300.054.783186
GGATAGT24500.054.5726
GATAGGT22350.054.4946827
GATAGGA31950.054.4424827
TATAGGG25900.054.322377
GCCGATA7250.053.586764
CTGATAG20550.053.4798745
CTATAGG14600.053.4594576
Produced by FastQC (version 0.11.5)