FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732376
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG10200.058.3349345
CGCGATA8250.058.122814
CCGATAC2550.057.648645
CGGGATA10700.057.244564
GGTATAG16950.056.3732265
GCGATAA11150.056.1898845
CGATAGT11100.056.1276636
GATAGGT18550.055.6598667
CGATAGG9950.055.5794146
GTATAGG17150.055.5117266
GGATAGT17850.055.491726
ACGATAG8400.055.418195
TATAGGC13100.055.3048367
GTGATAG17250.054.9870155
CCGATAA7450.054.9679575
GATAGGG17250.054.5792127
GCGGATA11350.053.966244
TATAGCG9350.053.903257
CGATATG11650.053.4778636
TATACGG7200.053.471737
Produced by FastQC (version 0.11.5)