FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3202979
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA8100.060.495594
GCGATAA13350.059.2526665
GGATAGG20750.058.7005126
CTATAGG14550.057.4930956
CGGGATA10400.057.2132074
GCGATAG13400.057.2031635
TCCCGTC10600.057.120749
CGGATAG12200.056.804945
CCTATAG9450.056.6683245
CGGCTAC4350.056.3234835
CGTATAG12700.056.2221155
GGTATAG17500.055.8016325
ACGATAG9600.055.782885
GGATAGT20600.055.389976
GATAGTC12650.055.334137
GCGTATA11600.055.217134
GTATAGG20550.055.0137756
CGATAAC6300.055.0016066
CGCTACC3000.054.8349386
GATAGGG20750.054.8174557
Produced by FastQC (version 0.11.5)