FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3629151
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC4450.059.7770235
CGGATAG12450.058.7567335
CAGATAG15150.058.4505465
CGCGATA9200.058.208224
CCGATAA10550.057.395045
CGGGATA11550.057.2743954
GCGATAA14050.057.0479245
CGTGATA14600.056.3372574
GCGATAG13050.056.3234835
CGATAGG13500.055.743136
GGATAGT23050.055.7290736
GTATAGG23950.055.534746
CGTATAC4800.055.4182785
CGATAGT12200.055.084336
ACGATAG10700.054.6277585
CCTGATA10000.054.601594
GCGTATA14500.054.3119284
GATAGTC13350.054.2697
CGCTATA12200.054.2228934
CGTATAG13500.054.186765
Produced by FastQC (version 0.11.5)