FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-40_HPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-40_HPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2778539
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCTTATAGGACTCCGCTGGCACCTTATGAGAAATCAAAGTTTTTGGG28090.10109629557116169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGGA42250.060.968497
GGATAGG18250.060.412316
TCCCGTC12300.060.323299
TGATAGG34100.060.0453346
GTATAGG20450.059.7323846
CTATAGG16200.059.6309626
CCTATAG8750.059.601335
CCGCTAC2600.059.2320945
CGTATAG10300.059.1275375
CCCGTCC15450.059.12434410
GCGTATA10050.058.508774
CGTATAC4200.058.334645
GATAGGG19250.058.1799777
GTCCCGT3600.057.35938
GGGATAG15950.057.0545655
CGCGATA6700.056.941574
CGTGATA10850.056.7754674
CGGATAG11050.056.69815
CAGATAG13150.056.4271165
GTATAGC7350.056.191736
Produced by FastQC (version 0.11.5)