FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2877771
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG11500.059.652355
CGTATAC3400.058.6766475
CAGATAG15550.057.170595
CGGGATA10950.056.8951454
TGATAGG22100.055.7477386
CGTATAG10750.055.6745875
GGATAGG21950.055.6503376
GGATAGT19150.055.562496
GGTATAG16200.055.5248575
GATAGGT20300.055.172587
GATAGGA25900.055.13537
GATAGGG18950.055.039747
GTGATAG18100.054.723925
ATAGGGC8850.054.576448
GCGATAA11050.054.4797975
GTATAGG17950.054.402226
CGGCTAC2850.054.0352485
TATACGG7650.053.9870957
CCGATAA9150.053.9345865
GATAGTC10450.053.9236077
Produced by FastQC (version 0.11.5)