FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2968248
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTCC6200.060.9680110
CCGCTAC2250.057.5577435
GGATAGT20400.056.4482236
CTATAGG11950.056.2364466
GGTATAG16850.055.6697735
TCCCGTC5900.055.169739
CGCGATA5800.054.9158824
GGATAGG19450.054.706456
CGTATAG9100.054.617465
GGGATAG16900.054.2624135
GATAGGT20850.054.2190327
CAGATAG11850.054.052695
CGGATAG10050.053.9821555
GTATAGG18500.053.9209756
GATAGGG19400.053.7613037
TGATAGG24450.053.253566
CGGGATA9050.052.9854434
GCGTATA9650.052.9553834
GCGATAA11050.052.8979385
GATAGGA25150.052.324517
Produced by FastQC (version 0.11.5)