FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684550
Sequences flagged as poor quality0
Sequence length76
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC3600.063.1970335
CGGATAG10700.061.8249635
GTATAGG21450.060.5397386
GCGATAA9500.060.4235275
CCTATAG7100.060.1433075
GATAGTC9550.059.7373287
GCGATAG10750.059.583835
CGATAGG12450.058.7585226
GGATAGC8550.058.54156
CGTATAG12800.058.5180245
TATAGGT29300.058.4120987
GATAGGA25600.058.2413187
GGATAGT15100.058.1822746
CAGATAG11750.058.087495
TATAGCG14200.057.9216737
GGTATAG16450.057.4491655
CGATAGT8900.057.4191556
CGCTACG3200.056.8783876
CCTATAA11900.056.7670335
TATACGG7300.056.57457
Produced by FastQC (version 0.11.5)