FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3099950
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAG5650.058.2314725
GGATAGG22100.058.123556
CGGGATA10250.056.684274
GCGATAG12000.056.2935
GGGATAG20650.056.1030245
GGATAGT23250.056.0013286
CGATAGT13550.055.7946786
GTATAGG21600.055.4179846
GATAGGT26250.055.3328637
GCCGATA7100.055.212584
CGTATAG9200.055.1643525
CGGATAG11150.054.306225
TATAGGG23400.054.1448367
CTATAGG10300.054.030416
TATAGGT31000.053.9672857
GGTATAG18550.053.5861785
TATAGCG9350.053.5289577
CAGATAG12900.053.1795545
CACGATA6650.053.159164
GTATAGT26800.052.3706446
Produced by FastQC (version 0.11.5)