FastQCFastQC Report
Tue 8 Dec 2020
HFHGWBGXG_n01_col4-0.5_LPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHFHGWBGXG_n01_col4-0.5_LPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2405200
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATAG11950.059.7537775
CGGGATA9300.057.961674
TATAGCG7300.057.0546577
CGGATAG8900.057.027085
GTATAGG15650.055.915066
GATAGGT14900.055.9059077
TGATAGG15100.055.865446
CGATAGG7650.055.82156
GGATAGT15950.055.7411776
CGTATAG7500.055.5378235
GCCGATA6200.055.3270534
CTGATAG12600.055.0044445
CCTGATA6750.054.96744
CGCGATA4400.054.89084
GCGATAG8250.054.7316935
GGTATAG15100.054.4745985
CGTATAC3500.054.004365
CTATAGG10500.054.004366
GCGATAA8950.053.970845
CCGATAC2400.053.9626925
Produced by FastQC (version 0.11.5)